HEPES-【7365-45-9】

Chinese name: 4-hydroxyethylpiperazine ethanesulfonic acid

English name: HEPES

CAS Number: 7365-45-9

Molecular formula: C8H18N2O4S

Molecular weight: 238.305

Appearance and smell: white crystalline powder

Melting/boiling point: 234-238℃

Content 99.5%

Specification: 25kg/barrel

Uses: Used in biochemical diagnostic kits, DNA/RNA extraction kits and PCR diagnostic kits. It has no toxic effect on cells, and is often used in the research of organelles and highly variable, pH-sensitive proteins and enzymes.

            It is a zwitterionic biological buffer, belonging to Good’s buffer, with an effective buffer range of 6.8~8.2. It can be widely used in a variety of biochemical reactions and used as a buffer reagent in certain cell culture media. The final concentration is 10~50mmol/L, and the buffering capacity can be achieved by generally containing 20mmol/L in the culture medium.